Titre | Multifunctional Peptide-conjugated hybrid silica nanoparticles for photodynamic therapy and MRI. |
Publication Type | Journal Article |
Year of Publication | 2012 |
Authors | Benachour, H, Sève, A, Bastogne, T, Frochot, C, Vanderesse, R, Jasniewski, J, Miladi, I, Billotey, C, Tillement, O, Lux, F, Barberi-Heyob, M |
Journal | Theranostics |
Volume | 2 |
Issue | 9 |
Pagination | 889-904 |
Date Published | 2012 |
ISSN | 1838-7640 |
Abstract | Photodynamic therapy (PDT) is an emerging theranostic modality for various cancer as well as non-cancer diseases. Its efficiency is mainly based on a selective accumulation of PDT and imaging agents in tumor tissue. The vascular effect is widely accepted to play a major role in tumor eradication by PDT. To promote this vascular effect, we previously demonstrated the interest of using an active- targeting strategy targeting neuropilin-1 (NRP-1), mainly over-expressed by tumor angiogenic vessels. For an integrated vascular-targeted PDT with magnetic resonance imaging (MRI) of cancer, we developed multifunctional gadolinium-based nanoparticles consisting of a surface-localized tumor vasculature targeting NRP-1 peptide and polysiloxane nanoparticles with gadolinium chelated by DOTA derivatives on the surface and a chlorin as photosensitizer. The nanoparticles were surface-functionalized with hydrophilic DOTA chelates and also used as a scaffold for the targeting peptide grafting. In vitro investigations demonstrated the ability of multifunctional nanoparticles to preserve the photophysical properties of the encapsulated photosensitizer and to confer photosensitivity to MDA-MB-231 cancer cells related to photosensitizer concentration and light dose. Using binding test, we revealed the ability of peptide-functionalized nanoparticles to target NRP-1 recombinant protein. Importantly, after intravenous injection of the multifunctional nanoparticles in rats bearing intracranial U87 glioblastoma, a positive MRI contrast enhancement was specifically observed in tumor tissue. Real-time MRI analysis revealed the ability of the targeting peptide to confer specific intratumoral retention of the multifunctional nanoparticles. |
DOI | 10.7150/thno.4754 |
Alternate Journal | Theranostics |
PubMed ID | 23082101 |
PubMed Central ID | PMC3475218 |